North Carolina State University researchers have developed a new technique that can alter plant metabolism reducing nicotine levels in tobacco plants, reports NC State University.

Tested in tobacco plants, the technique showed that it could reduce harmful chemical compounds, including some that are carcinogenic. “A number of techniques can be used to successfully reduce specific chemical compounds, or alkaloids, in plants such as tobacco, but research has shown that some of these techniques can increase other harmful chemical compounds while reducing the target compound,” said De-Yu Xie, professor of plant and microbial biology at NC State and the corresponding author of a paper describing the research. “Our technology reduced a number of harmful compounds – including the addictive nicotine, the carcinogenic N-nitrosonornicotine (NNN), and other tobacco-specific nitrosamines (TSNAs) – simultaneously without detrimental effects to the plant.”
The technique uses transcription factors and regulatory elements as molecular tools for new regulation designs. Regulatory elements are short, non-coding DNA fragments that control the transcription of nearby coding genes. Transcription factors are proteins that help turn certain genes on or off by binding to regulatory elements. Xie hypothesized that these could be useful molecular tools to design new regulations for engineering new plant traits, said the press release.
Xie and his colleagues tested the hypothesis by examining tobacco plants in the greenhouse and in the field and showed the reductions of harmful chemical compounds and nicotine in both types of experiments. NNN levels were reduced from 63 to 79 per cent in leaves from tobacco plants that had PAP1 and TT8 overexpressed. According to the press release, overall, four carcinogenic TSNAs were significantly reduced by the technique.
Xie believes that the technique holds the potential to be used in other crop plants to promote other beneficial traits and make some foods healthier. The paper appears in Journal of Advanced Research.